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KMID : 0359319960360020371
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Korean Journal of Veterinary Research
1996 Volume.36 No. 2 p.371 ~ p.378
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Isolation of Escherichia coli O157:H7 from animal feces and biochemical characteristics of Verotoxin - 2 produced by these strains
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Abstract
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Three and 2 strains of E coli O157 were isolated from fecal materials of cattle (390) and pigs (420) in Korea, respectively. One strain of O157:H7 isolated from cattle and 2 strains of O157:H7 isolated from pigs were identified as verotoxin-1 (VT-1) produing strains and 2 strains (O157:H7 and O157:H-) isolated from cattle were identified as verotoxin-2 (VT-2) producing strains by neutralization test on HeLa and Vero cells. Culture supernatants of the isolates were cytotoxic to HeLa and Vero cells. The levels of cytotoxin produced by isolates were 10²∼10⁴ cytotoxic dose(CD_(50))/㎖.
Also, VT-2-converting bacteriophage was isolated from KSC109 strain which had been isolated from cattle. Molecular weight of the phage DNA was determined as approximately 45 Kb in 0.8% agarose gel electrophoresis, and morphology of the phage stained with phosphotungstic acid was observed by transmissible electron microscopy.
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